Analysis of half diallel mating designs i a practical analysis procedure for anova approximation by g. The parents and hybrids were characterized for a wide number of traits of agronomic interest. Diallel analysis can be performed for data with unequal classifications, for different modeling situations viz. The published radiata pine data wu and matheson,2000 was analyzed to illustrate the convenience, flexibility and power of our software for diallel analysis.
Jan 21, 2018 the diallel cross analysis method proposed by hayman is particularly useful because it provides information, among others, on additive and dominance effects of genes, average degree of dominance, proportion of dominance, direction of dominance, distribution of genes, maternal and reciprocal effects, number of groups of genes that control a. Besides statistical analysis, this software performs mathematical, financial and scientific analysis. Genes is a software package used for data analysis and processing with different biometric models and is essential in genetic studies applied to plant and animal breeding. Diallel analysis for morphological and biochemical traits in tomato. Currently, we have no information on a widely available program in sas sas institute, 2014 to analyze a diallel cross data set from multiple locations or environments according to. A comprehensive program for griffings and gardnereberhart analyses yudong zhang, manjit s. Agdr is a set of r programs that performs statistical analyses to calculate diallel, line by tester, north carolina. It allows parameter estimation to analyze biological phenomena and is fundamental for the decisionmaking process and predictions of success and viability of selection strategies. Bayesian diallel analysis reveals mx1dependent and mx1independent effects on. A diallel cross is a mating scheme used by plant and animal breeders, as well as geneticists, to investigate the genetic underpinnings of qualitative and quantitative traits. A microcomputer program has been developed to be used as a teaching tool in quantitative genetics classes and as a means of analyzing real data from diallel crosses. We used a halfdiallel analysis, which gives information on the magnitude of general and specific combining abilities and trait heritabilities 2527, using 10 parents from different genetic backgrounds, origins, and morphological characteristics. In this technique, the total sum of squares is partitioned into various components, namely a. The procedure requires two runs of proc varcomp and results in estimates of.
From a genetical point of view, two assumptions are critical in attempts to interpret the resuits of diallel analyses. In this paper, we fully describe a sasbased software sashaydiall for performing a complete diallel cross analysis based on haymans model with or without reciprocals. Diallel is a greek word and introduced by schmidt in 1919. Usage griffingy, rep, cross1, cross2, data, method, model arguments y numeric response vector rep replicate as factor cross1 cross 1 as factor cross2 cross 2 as factor data a ame method method for diallel analysis using grif. Bglr provides predictions, gwas analysis and analysis of reaction norm model described in reference 1. Sheoran, computer programmer at ccs hau, hisar, india. Contribute to myaseen208diallelanalysisr development by creating an account on github.
Th\as, the purpose of the analysis of the full diallel cross is to investigate the types and magnitudes of variability that contribute to differences among the p inbred homozygous parental lines. Heterosis was determined as outlined by foohad and bassiri 1983. Statistical packages icarindian agricultural statistics. Sashaydiall is a code written in sas to perform diallel analysis according to the method of hayman 1954. Introduction griffings analysis hayman and jinks analysis variety cross diallel partial diallel cross discussion statistical programssoftware summa. The lack of easytouse specialized software for the hayman 1954b diallel analysis method limits its application in plant breeding. Dial is a computer package for the analysis of full and half dialel table of a quantitative trait. Sughroue iowa state university follow this and additional works at. K ing 2 received 23rd june 1997 abstract procedures to analyze halfdiallel mating designs using the sas statistical package are presented. Jul 18, 2018 agdr is a set of r programs that performs statistical analyses to calculate diallel, line by tester, north carolina. Opstat provides the analysis of diallel crosses laid out in randomised block design using griffings 1956 approach all four methods. For proper recommendation of the most reliable method of effective crop improvement, the knowledge and practice of diallel analysis is paramount. Diallel analysis and separation of genetic variance.
Diallel analysis and its applications in plant breeding. Agdr contains a graphical java interface that helps the user to easily choose input files, which analysis to implement, and which variables to analyze. Hayman is used for performing diallel analysis using haymans approach. Agdr analysis of genetic designs with r for windows. The sashaydiall program was written in sasiml and runs in sas version 9. And after installing it, you need to load it in the r session. There are four main types of diallel mating design.
The data were further subjected to the diallel analysis proposed by griffing 1956, method 2, model 1 all possible combinations excluding reciprocals to determine the combining ability to separate some components of genetic variance. From a statistical point of view the critical issue concerns the choice of a model with fixed or random genotypic effects. Hayman diallel analysis using hayman approach description hayman is used for performing diallel analysis using haymans approach. Application of diallel analyses in crop improvement. Diallel cross refers to mating of selected parents in all possible combinations and evaluation of a set of diallel crosses is known as diallel analysis. Some diallel mating designs are north carolina designs, line by tester designs and diallel designs. Are there free available softwares for diallele analysis. Usage haymany, rep, cross1, cross2, data arguments y numeric response vector rep replicate as factor cross1 cross 1 as factor cross2 cross 2 as factor data a ame details diallel analysis using haymanss. Griffing 1956 provided clear statements of methods of analysis of diallel crosses in terms of general and specific combining ability and the circumstances in which each method of analysis should be used. Griffings diallel analysis is used in plant improvement programs to identify superior parents for crossing and for characterizing general, specific, and reciprocal effects.
Griffing is used for performing diallel analysis using griffings approach. Department of statistics, university of nebraska lincoln, ne, usa. Description performs diallel analysis with r using griffings and haymans approaches. A real example is given to illustrate the convenience, flexibility and power of our software for diallel analysis. A comparison of different diallel analyses springerlink. Griffing is used for performing diallel analysis using grif. Genstat programs for haymans approach of diallel analysis in two parts have been developed. Gsca can perform analyses not only for griffings four types of diallel crosses but also for more complicated diallel crosses whether the data structure is balanced or unbalanced. New software and algorithms to analyse diallel mating. Our department produced two software for analysis hyman and griffing. The sashaydiall program requires an input csv format data file that has replication, parent 1, parent 2, and the trait of interest for a single site experiment. There is beta package plantbreeding, which can do diallel analysis.
Diallel genetic analysis for multiple traits in eggplant and. The diallel cross analysis method proposed by hayman is. I think its unlikely that youll find worked examples in r for the analysis of diallels. Department of mathematics and statistics, university of agriculture faisalabad, pakistan. This video deals with line x tester analysis in r software of 5 lines 3 testers with 4 replications. Five different methods of diallel analysis have been compared using data from a half diallel cross of a fixed set of nine homozygous varieties and one set of their single cross progenies in chickpea.
The first program will carryout the analysis of variance. Performs diallel analysis with r using griffings and haymans approaches. Crop science abstract issues in diallel analysis digital. Random effects model can be applied using griffings approach. Eight different modelmethod combinations are commonly used in the analysis. The accuracy of the analysis is improved by using the appropriate model and method. Bglr is a software to simplify the selection of input files and parameters to perform bayesian generalized linear regression using r statistacal software. Jan 30, 2019 this video deals with line x tester analysis in r software of 5 lines 3 testers with 4 replications.
Diallel mating designs are generally used to obtain estimates of genetic effects for a fixed set of parental lines fixed effects or to estimate general combining ability gca and specific combining ability sca variance components from a set of. I did find some references for diallel analysis in sas e. Dial is a computer package for the analysis of full and half dialel table of a. Opstat is a free online agriculture data analysis tool created by o. Principles for griffings combining ability analysis. Diallel mating design is used to evaluate several inbred lines in terms of combining ability variances and effects. In this technique, the total sum of squares is partitioned into various components, namely a additive, b nonadditive, wich is further subdivided into b1, b2 and b3, c maternal and d reciprocal differences other than c. The experimental design discussed is the randomized complete block design with replication. There are some packages which deals with diallel analysis agricolae. Genes a software package for analysis in experimental statistics and quantitative genetics. Proper analysis of the diallel mating design jay r.
Different methods of diallel crossing are commonly used in plant breeding. Statistical software is a specialized computer program for easy analysis of statistical problems in an innovative way. Eloquent information about the genetic basis of inheritance is important for any breeding program. To put the result into practice, we have developed a windows software program gsca for analyzing a flexible diallel linear model that could contain the gca, sca, reciprocal, block and environment effects as well as interaction effects such as gca by environment. Generally, university students, teachers and researchers use statistical analysis software. Therefore, a diallel study was conducted under the influence.
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